Rapid Enrichment and High-sensitivity Detection of Melamine from Milk with Immunomagnetic Microspheres
نویسندگان
چکیده
One of the key concerns of the people and the government of China is food safety. After the use of melamine as an additive was reported in 2008, melamine detection was required in milk. The current methods to detect and confirm melamine are mainly HPLC and HPLC-MS/MS, but the pre-treatment processes include some tedious steps making them time-consuming and environmentally unfriendly. In contrast, the on-line detection of melamine utilizing colloidal gold test paper was appreciated, but its sensitivity has not yet complied with the demands. Here, we report a novel and rapid method for enriching the melamine content of milk using immuno-magnetic microspheres, which may allow the 100fold concentration of melamine within 20 min. The combination of these methods allowed a decreased detection time, an improved detection efficiency of LC-MS/MS and improved the sensitivity of on-line melamine detection, helping milk processing factories to detect melamine in their products.
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